Zorbax Bonus-RP

Zorbax Bonus-RP Guard Columns from Agilent Technologies

Choosing to use guard columns can help reduce operating expenses, by reducing the frequency of analytical column replacement. The guard column prevents damage caused by particulate matter and strongly adsorbed material. To maintain an adequate capacity for sample impurities, choose a guard column with an internal diameter similar to the column internal diameter.

Ideally, the packing of the guard column should be the same as the analytical column so that the chromatography of the analytical column is not altered. Guard columns contribute to the separation, so you should include a guard column inline during method development. Agilent UHPLC guards provide protection for high-efficiency Poroshell 120and ZORBAX RRHD and RRHT columns, without reducing performance.

Bonus-RP columns provide unique selectivity and applicability for mid pHs with an embedded amide linkage in the C14-alkyl chain. At pH 7 Bonus-RP provides excellent peak shape for even the most challenging basic compounds. Bonus-RP is protected from hydrolysis at low pH using the same sterically protecting bulky side chains as StableBond, and also is triple endcapped to minimize silanol interactions and improve stability at mid pH. Bonus-RP can be used without phase collapse in 100% aqueous mobile phases.