Zorbax 300StableBond

Zorbax 300StableBond Guard Columns from Agilent Technologies

Choosing to use guard columns can help reduce operating expenses, by reducing the frequency of analytical column replacement. The guard column prevents damage caused by particulate matter and strongly adsorbed material. To maintain an adequate capacity for sample impurities, choose a guard column with an internal diameter similar to the column internal diameter.

Ideally, the packing of the guard column should be the same as the analytical column so that the chromatography of the analytical column is not altered. Guard columns contribute to the separation, so you should include a guard column inline during method development. Agilent UHPLC guards provide protection for high-efficiency Poroshell 120and ZORBAX RRHD and RRHT columns, without reducing performance.

Agilent ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons. Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases. For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers.