AdvanceBio Oligonucleotides

AdvanceBio Oligonucleotides Guard Columns from Agilent Technologies

Choosing to use guard columns can help reduce operating expenses, by reducing the frequency of analytical column replacement. The guard column prevents damage caused by particulate matter and strongly adsorbed material. To maintain an adequate capacity for sample impurities, choose a guard column with an internal diameter similar to the column internal diameter. Ideally, the packing of the guard column should be the same as the analytical column so that the chromatography of the analytical column is not altered.

To successfully separate trityl-off, deprotected olingonucleotides, you need columns that have high resolving power and are robust enough to withstand challenging conditions. If your columns don't provide enough resolution, you can't be sure your results are accurate. If your columns are not robust, you will have to replace them frequently, which will disrupt your workflow and increase your costs.